Fig. 5: Demethylation of C1QTNF6 Enhances Its Expression, Promoting Proliferation and Invasion in Bladder Cancer. | npj Precision Oncology

Fig. 5: Demethylation of C1QTNF6 Enhances Its Expression, Promoting Proliferation and Invasion in Bladder Cancer.

From: Bladder cancer diagnostic and prognostic models from DNA methylation by multi algorithm machine learning

Fig. 5: Demethylation of C1QTNF6 Enhances Its Expression, Promoting Proliferation and Invasion in Bladder Cancer.The alternative text for this image may have been generated using AI.

A qPCR analysis of 11 target gene expression levels. B DNA methylation levels of the 11 target genes in SV-HUC-1 versus 5637 cell lines. C Western blotting analysis confirming C1QTNF6 upregulation in bladder cancer cell lines (P < 0.01). D Downregulation of C1QTNF6 expression in the 5637 cell line (P < 0.01). E, F Functional assays demonstrating that C1QTNF6 downregulation reduces proliferation (E, CCK-8 assay, P < 0.001) and weakens invasion ability (F, Transwell assay, P < 0.01). G DNA methylation levels of C1QTNF6 were increased (P < 0.05). I Upregulation of C1QTNF6 DNA methylation suppressed its expression (Western blotting, P < 0.01). J, K Increased DNA methylation of C1QTNF6 inhibited proliferation (J, CCK-8 assay, P < 0.001) and invasion (K, Transwell assay, P < 0.01) in bladder cancer cell lines.

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