Fig. 2: Characterization of CRC candidates in Ewing sarcoma.
From: The hegemonic EWSR1::ETS oncoprotein overrules core regulatory circuitry principles in Ewing sarcoma
A Heatmap showing the 12 genes predicted to be specific to Ewing sarcoma CRC (≥50% of the Ewing sarcoma cell lines) compared to control data (<30%). Each row represents a gene, and each column a sample. Black and white boxes represent respectively the presence and the absence of the gene in the CRC. The code color at the top is the same as in Fig. 1A. B Spearman correlation plot of expression of 132 Ewing sarcoma tumors for the 12 candidate TFs. C Boxplot of log2 fold change for the 12 previous candidate genes in each of the seven Ewing cell lines expressing EWSR1::ETS compared to EWSR1::ETS-KD oncogene (RNA-Seq data). D Heatmap of the number of FLI1/ERG mSat peaks in super-enhancer (SE) associated with the 12 candidate TF. NA values correspond to TFs without an associated SE in the given cell line. E Heatmap of position of FLI1 mSat peaks in enhancer-promoter chains in A-673 and TC-71 for the 12 candidate TFs (H3K27ac-HiChIP)9. F Venn diagram showing the selection of CRC candidates, corresponding to six MTFs that meet at least two of the three criteria (gray area).
