Fig. 5: Concomitant silencing of all candidate Master transcription factors (MTFs) is not sufficient to alter the proliferation of Ewing sarcoma cells. | npj Precision Oncology

Fig. 5: Concomitant silencing of all candidate Master transcription factors (MTFs) is not sufficient to alter the proliferation of Ewing sarcoma cells.

From: The hegemonic EWSR1::ETS oncoprotein overrules core regulatory circuitry principles in Ewing sarcoma

Fig. 5: Concomitant silencing of all candidate Master transcription factors (MTFs) is not sufficient to alter the proliferation of Ewing sarcoma cells.

A, B RT-qPCR of MTFs and EWSR1::FLI1 gene expression in EW-1 (A) and TC-71 (B) upon pooled silencing of all MTFs (siPool) compared to siRNA control conditions (siCT) (n = 3). C, D Proliferation rates in EW-1 (C) and TC-71 (D) cells measured using Incucyte technology after siPool compared to siCT (n = 3). EW-1 (E) and TC-71 (F) cell counts upon siPool compared to siCT (n = 3). Data were shown as mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed Student’s t-test); ns not significant. EW-1 data shown 72 h post-transfection, and TC-71 data shown 48 h post-transfection.

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