Fig. 3: The spatiotemporal differential analysis of CD4+ T cells for the identified targets. | npj Precision Oncology

Fig. 3: The spatiotemporal differential analysis of CD4+ T cells for the identified targets.

From: Transcriptome-wide Mendelian randomization and single-cell analysis during CD4+ T cell activation deciphers immunotherapeutic targets for colorectal cancer

Fig. 3: The spatiotemporal differential analysis of CD4+ T cells for the identified targets.The alternative text for this image may have been generated using AI.

A Illustration of the spatiotemporal differential analysis framework. B The t-SNE visualization of CD4⁺ T cell subclusters identified by scRNA-seq. C–F The t-SNE and volcano plots of identified targets in the SD group pre- and post-immunotherapy. Gray dots represent non-differentially expressed genes. SD steady disease, CR complete response, PR partial response. G Biological functions associated with clusters 3, 7, and 9, compared to cell subpopulations with low expression of ORMDL3, PARP14, and KCNA3. H T-cell dysfunction scores predicted using the TIDE database for high and low expression groups of identified targets. Statistical significance is indicated as *p < 0.05, **p < 0.01, ***p < 0.001.

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