Fig. 4: RICH1 overexpression in breast cancer cells affects the polarization of macrophages M1 by secreting IFN-γ and chemokines. | npj Precision Oncology

Fig. 4: RICH1 overexpression in breast cancer cells affects the polarization of macrophages M1 by secreting IFN-γ and chemokines.

From: RICH1 enhances pro-inflammatory TAM infiltration in breast cancer via promoting TRIM21-mediated ubiquitination of RhoA and inhibiting STAT3 phosphorylation

Fig. 4: RICH1 overexpression in breast cancer cells affects the polarization of macrophages M1 by secreting IFN-γ and chemokines.

A LogFC of mRNA expression level of chemokines, which polarize macrophages towards M1 or M2 in 4T1-RICH1 OE/NC. B The secretion level of IFN-γ, CXCL9, IL-4, and IL-13 was evaluated through ELISA between 4T1-RICH1 OE/NC. C The PCR products by RICH1 primers were analyzed by electrophoresis in agarose gels stained with ethidium bromide, which were extracted from HC11-RICH1 K/O1/2/ NC established using CRISPR/Cas9 RICH1/NC-targeted lentivirus and picking monoclonal. D The secretion level of IFN-γ, CXCL9, IL-4, and IL-13 was evaluated through ELISA between HC11-RICH1 K/O1/2/ NC. E The volume and weight of the tumors compared in the mice injected with 4T1-RICH1 OE shIFNG/4T1-RICH1 OE. F The proportions of CD86+ subpopulations in CD45+CD11b+F4/80+ cells of TME in the above tissues by flow cytometry analysis. Abbreviation: FC fold change, ELISA enzyme linked immunosorbent assay, PCR polymerase chain reaction. P values in (A, B, D, E and F) were calculated using unpaired two-tailed Student’s t tests. *P < 0.05; **P < 0.01; ***P < 0.001.

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