Fig. 2: Knockdown of KRAS significantly increased the infiltration of cytotoxic T lymphocytes and restored the level of cancer cell-intrinsic STING.

A The subset of tumor-infiltrating cytotoxic IFNγ⁺CD8⁺ cells within primary tumors was examined by flow cytometric analysis. The ratio of IFNγ⁺CD8⁺/Foxp3⁺ Treg was shown (n = 3-4). One-way ANOVA t test. *p < 0.05. B The resected primary tumors were homogenized to evaluate type I signatures by qRT-PCR (n = 3-4). One-way ANOVA t test. *p < 0.05 and **p < 0.01. C The proliferation marker Ki67, tumor STING, GzmB⁺ immune cells and CD11c⁺ DCs were evaluated by immunohistochemistry (n = 3). D The quantification of tumor STING (H-score), GzmB⁺ immune cells and CD11c⁺ DCs (n = 3). One-way ANOVA t test. *p < 0.05 and **p < 0.01. E The resected primary tumors were homogenized for immunoblotting analysis (n = 3). One-way ANOVA t test. **p < 0.01. F The non-irradiated distant tumor volume (abscopal tumor) was measured every 3 days (n = 4–6). The resected abscopal tumors on Day 28 were weighted (n = 4-6). One-way ANOVA and two-way ANOVA t test. *p < 0.05, **p < 0.01 and ***p < 0.001. G The subset of tumor-infiltrating CD4⁺ cells, CD8⁺ cells, MDSCs (Gr1⁺CD11b⁺) and Treg (CD4⁺CD25⁺Foxp3⁺) within abscopal tumors was evaluated by flow cytometric analysis (n = 3-4). One-way ANOVA t test. *p < 0.05. H The proliferation marker Ki67 and CD8⁺ immune cells were evaluated by immunohistochemistry (n = 3). I The quantification of Ki67⁺ cells was shown (n = 4). One-way ANOVA t test. *p < 0.05. J The quantification of CD8⁺ immune cells was shown (n = 4). One-way ANOVA t test. *p < 0.05. K The resected abscopal tumors were homogenized to evaluate Ifnb1 mRNA by qRT-PCR (n = 3-4). One-way ANOVA t test. *p < 0.05 and **p < 0.01. L The resected abscopal tumors were homogenized to evaluate Cxcl10 mRNA by qRT-PCR (n = 3-4). One-way ANOVA t test. *p < 0.05 and **p < 0.01.