Fig. 2: Pre-steady-state kinetics analysis. | Nature Catalysis

Fig. 2: Pre-steady-state kinetics analysis.

From: Bypassing evolutionary dead ends and switching the rate-limiting step of a human immunotherapeutic enzyme

Fig. 2

a–d, Stopped-flow, pre-steady-state fluorescence traces of OH-AA (first product of the reaction) formation with 25 μM HsKYNase with 800 μM KYN (a), 5 μM HsKYNase_66 with 500 μM KYN (b), 5 μM HsKYNase with 500 μM OH-KYN (c) and 12.5 μM HsKYNase_66 with 1000 μM OH-KYN (d). Each trace represents the average of five different experiments. The red traces include all data points. The black line shows the fit to 1 − the exponential burst equation (equation (2) in the Methods).

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