Extended Data Fig. 3: Catalytic activity of SdnG and K127X mutants.
All reactions were carried out for 1 min with 100 µM 1. Values and error bars are obtained from the average and standard deviation of three independent measurements (black circles) respectively (n = 3). (a) Absolute rates of non-enzymatic and enzymatic DA cyclization of 1. Asterisks indicate no measurable substrate consumption during the course of the reaction. (b) Relative activity of SdnG and K127X variants normalized by enzyme concentration. All activities are shown relative to the rate acceleration of the DA reaction exhibited by the wild-type enzyme (WT, 100%). A 0% value (asterisks) indicates no rate acceleration compared to uncatalyzed DA reaction.
