Fig. 5: Reverse engineering based on transcriptome and whole-genome re-sequencing analyses.
From: Mutations in hik26 and slr1916 lead to high-light stress tolerance in Synechocystis sp. PCC6803
a PCC6803, b Tol, c Tol(S1), d 6803/OE-isiA, e 6803Δslr1916, f Tol(S1)Δslr1916, g 6803Δslr1916/slr1916m, h 6803Δhik26, i Tol(S1)Δhik26, j 6803Δhik26/hik26m strains. Culture under a light intensity of 40 μmol m−2 s−1 was performed using an LED plate, and cultures under other light intensities (4000, 7000, and 9000 μmol m−2 s−1) were performed using point source LED. The filled and open circles represent averages and individual data points in the biological triplicate experiments. Error bars indicate standard deviation from triplicate experiments. Note that the errors of the growth curve of the 6803Δhik26 strain under 4000 μmol m−2 s−1 were large because in the triplicate experiments similar growth as that of PCC6803 was observed in one culture experiment and severe-inhibited growth was displayed in remaining two culture experiments. In the 6803Δslr1916/slr1916m and 6803Δhik26/hik26m strains, the mutated genes were introduced at the neutral sites, not at their natural positions.
