Fig. 2: Phosphorylated parkin variants show similar levels of autoubiquitination as wild-type.
From: Naturally occurring hyperactive variants of human parkin
a Time-course of the autoubiquitination activity of phosphorylated GST-parkin with pUb bound. The decrease of unmodified parkin band present at different times of reaction is shown below the gel. b Quantification of the decrease in unmodified phosphorylated GST-parkin:pUb over time and S.E.M. from three replicate experiments (n = 3). The activity levels of the controls (pWT and pW403A) are shown only on the last graph for clarity.
