Fig. 8: Quantification of GFP⁺ donor-derived cells in the CNS revealed various trends across gestational ages.

a Violin plot of total CNS GFP+ cells/mm³ as compared to the gestational age at the time of injection. Peak cell concentrations reached in E13–E14 b FB and HB concentrations for each gestational age (mean with SD), with E13–E14 once again being the peak gestational age concentration; c GFP⁺ cellular concentrations were much higher within the HB as compared to FB (all samples across all gestational ages, paired t test, p < 0.0001, plotted as mean with SD); d–f there was no relationship between either the lifetime average PB chimerism or the chimerism at the time of harvest with regard to the cell concentration, without trend when analyzed by gestational age at the time of injection. g Analysis of cell concentration over time showed the highest level of cells present at 12 months of age (Kruskal–Wallis one way ANOVA, p = 0.0236 as compared to nadir at 6 months, plotted as mean with SEM); h PB chimerism decreased over time (mean with SEM). Linear trend ANOVA revealed a decrease of 1.85% each month. i There was a marked increase in peripheral blood chimerism with later gestation treatment, which reached statistical significance in each group (paired one-way ANOVA, plotted as mean with SEM). FB forebrain, HB hindbrain, PB peripheral blood. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All source data for (a–c, g) can be found in Supplemental Data 1. All source data for (d–f, h, i) can be found in Supplemental Data 2.