Table 3 Primary antibodies used for immunofluorescence and RNAScope staining
Primary Antibody | Cells Labeled | Antibody type | Concentration | Vendor |
|---|---|---|---|---|
GFP | Donor-derived | Rabbit anti-mouse | 1:10,000 (free floating) | NovusBio, NB600-308B |
GFP | Donor-derived | Rabbit polyclonal | 1:1000 | Abcam, ab290 |
Iba-1 | Microglia, meningeal macrophages | Rabbit polyclonal | 1:1000 | Fujifilm Wako Chemicals, 019-19741 |
GFAP | ENS glia, astrocytes | Rabbit polyclonal | 1:200 (slide mounted) 1:2000 (free floating) | Agilent Dako, Z0334 |
Calbindin | Purkinje neurons | Rabbit monoclonal | 1:1000 | Abcam, ab108404 |
Neu-N | Mature Neurons | Mouse polyclonal | 1:100 | EMD Millipore, MAB377 |
SOX2 | Stem/Progenitor cells | Rabbit monoclonal | 1:200 | Abcam, ab92494 |
HuC/HuD | ENS neuron | Human monoclonal | 1:10,000 (free floating) | Laboratory of Vanda Lennon, ANNA-1 |
eGFP | Donor-derived | Polyclonal | 1:50 | Advanced Cell Diagnostics, 492018 |
SRY | Male chromosomal marker | Mouse monoclonal | 1:50 | Advanced Cell Diagnostics, 552688 |
RBFOX3 | Gene coding for NeuN | Mouse monoclonal | 1:50 | Advanced Cell Diagnostics, 52897 |
