Fig. 3: Inhibition of CYP19A1 transcription by CTBP1 is rescued by FSH and LH. | Communications Biology

Fig. 3: Inhibition of CYP19A1 transcription by CTBP1 is rescued by FSH and LH.

From: CTBP1 links metabolic syndrome to polycystic ovary syndrome through interruption of aromatase and SREBP1

Fig. 3: Inhibition of CYP19A1 transcription by CTBP1 is rescued by FSH and LH.

A Firefly/Renilla luciferase activity ratios tested by co-transfection of vector or CTBP1 cDNA along with pGL3 basic vector and pGL3-CYPT19A1-II plasmid in HEK293T cells. B CYP19A-II promoter fragments detected by ChIP-qPCR using CTBP1 antibody or isotypical IgG, and the primer pairs 1-2. C, D PCR products detected by agarose gel electrophoresis (C) and sequencing (D). E–J CTBP1, aromatase, and E2 expression detected by qRT-PCR (E, H), WB (F, I) or ELISA (G, J) in KGN cells stably expressing either vector or CTBP1 cDNA after treatment with FSH (E–G) or LH (H–J) at 400 ng/ml. Quantification data are presented as mean ± SD and analyzed using two-tailed t-test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 are considered significant. pGCs Primary granulosa cell, WB Western blot.

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