Fig. 1: The B-body in quiescent primary oocytes characterized by EM and antibody staining.
From: The mouse Balbiani body regulates primary oocyte quiescence via RNA storage
A An EM image showing the cross-section of a B-body (arrow) in the quiescent primary oocyte at low-magnification. A’ A high-magnification EM image showing the cross-section of a B-body - highly stacked Golgi complexes in a circular structure. B A B-body stained by Golgi cis-face marker GM130 in a VASA-positive quiescent primary oocyte. B’ 3-D reconstructed image showing a B-body in a spherical structure. Co-immunostaining of the B-body by using GM130 and TGN46 (C), COPII (D), Clathrin (E), RNA green dye (F), PABPC (G), 7-methylguanosine (H), MIWI (I), MILI (J), AGO2 (K), EIF4E (L), ribosomal protein S6 (M), FMRP (N), GW182 (O), DDX6 (P), DCP2 (Q), DCP1A1-350 (R), DCP1A500-C (S), DCP1A300-400 (T), and mRNA-capping enzyme (mRNACE) (U). The antibody staining was repeated at least three times independently, and minimum n = 60 oocytes were examined to ensure that consistent staining results were observed. Confocal images from a single representative optical section are shown in the figure.
