Fig. 3: B-body disassociation activates primordial follicles.
From: The mouse Balbiani body regulates primary oocyte quiescence via RNA storage
A Schemes of neonatal mouse ovary culture and mouse injection. B Immunostaining of GM130 showing the Golgi complex morphology after a 2 h-drug treatment (arrowheads: B-bodies; arrows: disassociated B-bodies). C Percentages of primary oocytes containing a B-body in the ovaries after a 2 h drug treatment. D Ovarian morphology of drug-treated ovaries after 6 days culture. E Numbers of developing follicles in each drug-treated ovary after 6 days culture. F Numbers of developing follicles in each P10 ovary after the drug injection. Nascent protein synthesis revealed by fluorescent positive O-propargyl-puromycin (OPP) foci in the ovaries treated with cytochalasin D (cytoD) for 2 h (G–K) and cytochalasin D plus cycloheximide (cyclo) for 2 h followed by cycloheximide for 1 h (L). M Numbers of developing follicles in each drug-treated ovary after 6 days culture. Drug treatment in cultured ovaries and injection were repeated three times independently. N = 60 oocytes were examined for OPP distribution. Confocal images from a single representative optical section are shown in the figure. Quantification results were analyzed statistically by using one-way ANOVA and shown as Mean ± SD, P < 0.05 was considered statistically significant *.
