Fig. 4: Profiles of B-body-associated mRNAs enriched by RNA immunoprecipitation with DCP1A.
From: The mouse Balbiani body regulates primary oocyte quiescence via RNA storage
A Western blot image showing proteins pulled down by the DCP1A300-400 antibody. Rabbit (Rb) IgG was used as the negative control. IgG heavy chain (~50 kDa) was detected in both antibody pull-down (PD) samples. DCP1A protein (~74 kDa) was detected in primordial follicle protein lysis (input), Rb IgG unbounded (UB) sample and DCP1A pull-down sample. B Volcano plot showing enriched mRNAs in the DCP1A pull-down sample or in the cytoplasm. C In situ hybridization images showing the expression and location of RNAs that are highly enriched in DCP1A pull-down mRNAs. The probe to DapB was used as the negative control. (n = 60 oocytes). D Protein expression (normalized spectral count) of DCP1A pull-down mRNAs and cytoplasm-enriched mRNAs in quiescent primordial follicles (QF) and developing follicles (DF). E, F Gene ontology analysis results showing the top 10 candidates of the molecular function (E), biological process (F), REACTOM (G) of DCP1A pull-down mRNAs. The RNA-IP and in situ hybridization were repeated three times independently.
