Fig. 5: B-body may function downstream of FOXO3a-mediated primordial follicle activation.
From: The mouse Balbiani body regulates primary oocyte quiescence via RNA storage
A–E The expression and distribution of FOXO3a in primary oocytes in P4 ovaries. White arrows indicate developing oocytes (A) and primary oocytes (in B–E) with FOXO3a nucleus negative staining. (F, F’) Percentages of primary oocytes with (w) or without (w/o) a B-body that were FOXO3a nucleus positive (FOXO NU + ) or negative (FOXO NU-). G–I The expression and distribution of FOXO3a in control, cytochalasin D-, and bpV(pic)-treated ovaries. J, J’ Percentages of primary oocytes with a B-body that were FOXO NU + or FOXO NU- in cultured ovaries. K, K’ Percentages of primary oocytes without a B-body that were FOXO NU + or FOXO NU- in cultured ovaries. F’, J’, K’ show the individual data points for the same data as in (F, J, K). The numbers in (F, J, K) represent the percentages of FOXO NU+ primary oocytes. L The proposed model of primary oocyte quiescence and activation regulated by B-body-mediated RNA storage. Quantification results were analyzed statistically by using unpaired t-test and shown as Mean ± SD, P < 0.05 was considered statistically significant *.
