Fig. 3: Bacterial hyperpolarization effects of AMPs at subinhibitory concentrations.
From: Chemically diverse antimicrobial peptides induce hyperpolarization of the E. coli membrane
The flow cytometric membrane polarisation assay (BacLight) was carried out in E. coli using the fluorescent membrane potential indicator dye DiOC2(3). Representative histograms of the log ratio calculated from the red and green channels for LL37 (a) MAG2 (b) and AP (c) (yellow untreated, blue AMP treated). Population means of the red/green fluorescence ratios normalised to the values obtained for the untreated cells (d). Data are based on three biological replicates measured at 0.5 MIC AMP concentrations. Ratios below one correspond to depolarisation, and values above one indicate hyperpolarization. Values are displayed against the inducibility of the secondary structure. AMP-induced bacterial hyperpolarization detected for hyperpolarising sequences at concentrations of MIC of 0.25 (green), 0.5 (grey) and 0.75 (brown), n = 3 (e).
