Fig. 3: Anion selectivity and sensitivity of mNeonGreen, mNG-C149Y/W167Y, and Thyone.

a–c The fluorescence changes of mNeonGreen, mNG-C149Y/W167Y, and Thyone upon titration against selected anions at pH 7.4 (n = 2 independent experiments each; data points, means; error bars, SD). d Kd values of mNeonGreen, mNG-C149Y/W167Y, and Thyone to respective anions calculated based on the data shown in (a–c) (n = 2 two independent experiments each; bar graphs, mean).