Fig. 4: STUB1 suppressed cell proliferation and induced ferroptosis of PTX-resistant OC cells by downregulating HOXB3 expression. | Communications Biology

Fig. 4: STUB1 suppressed cell proliferation and induced ferroptosis of PTX-resistant OC cells by downregulating HOXB3 expression.

From: STUB1 suppresses paclitaxel resistance in ovarian cancer through mediating HOXB3 ubiquitination to inhibit PARK7 expression

Fig. 4

a, b HOXB3 expression was examined in sh-HOXB3-transfected SKOV3/R and A2780/R cells by RT-qPCR and western blot. sh-STUB1 with/without sh-HOXB3 was transfected into SKOV3/R and A2780/R cells upon PTX treatment. c Cells viability was examined using CCK-8. d Cells proliferation was evaluated using clone formation assay. e Mitochondrial morphology was monitored using a TEM. f–i ROS, iron, MDA and GSH were detected using suitable kits. j PARK7 and GPX4 levels were determined using western blot. N = 3. *P < 0.05; **P < 0.01, ***P < 0.001.

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