Fig. 2: CpY binding site. | Communications Biology

Fig. 2: CpY binding site.

From: Structural mechanisms of potent lysophosphatidic acid receptor 1 activation by nonlipid basic agonists

Fig. 2

a Surface representation of the ligand binding pockets in LPA1. CpY and LPA (18:1) (PDB 7TD0) are represented by stick models. b Surface charge of the binding pocket. Structure diagram (c), and schematic representation (d) of the interactions between CpY and the receptor within 4.5 Å. The dashed lines indicate the salt bridge with D1293.33 and hydrogen bonds, and the line indicate the CH–π interaction. e NanoBiT-G-protein dissociation assays for LPA1 and its mutants. Concentration–response curves of CpY-dependent G-protein dissociation signals for LPA1 are shown as means ± s.e.m. Individual replicates are shown as small dots. f Conservation of the CpY binding site in LPA1-3.

Back to article page