Table 1 Cryo-EM data collection, refinement and validation statistics
Data collection | CpY-LPA1-Gi-scfv16 | |
|---|---|---|
Microscope | Titan Krios (Thermo Fisher Scientific) | |
Voltage (keV) | 300 | |
Electron exposure (e−/Å2) | 50.362 | 48.405 |
Detector | Gatan K3 summit camera (Gatan) | |
Magnification | ×105,000 | |
Defocus range (μm) | −0.8–1.6 | −0.6–1.6 |
Pixel size (Ã…/pix) | 0.83 | |
Number of movies | 5013 | 3312 |
Symmetry | C1 | |
Picked particles | 3,667,591 | 2,393,563 |
Final particles | 277,598 | |
Map resolution (Ã…) | 2.86 | |
FSC threshold | 0.143 | |
Model refinement | ||
Atoms | 8,779 | |
R.m.s. deviations for ideal | ||
Bond lengths (Ã…) | 0.004 | |
Bond angles (°) | 0.553 | |
Validation | ||
Clashscore | 8.03 | |
Rotamers (%) | 0.11 | |
CCmask | 0.69 | |
EMRinger score | 2.32 | |
Ramachandran plot | ||
Favored (%) | 96.96 | |
Allowed (%) | 3.04 | |
Outlier (%) | 0 | |
