Fig. 10: MAZ regulation of mitochondrial function promotes malignant progression of melanoma. | Communications Biology

Fig. 10: MAZ regulation of mitochondrial function promotes malignant progression of melanoma.

From: Molecular mechanisms of MAZ targeting up-regulation of NDUFS3 expression to promote malignant progression in melanoma

Fig. 10

a For mitochondrial respiration, the oxygen consumption rate (OCR) of melanoma cells with MAZ overexpression and knockdown was determined using a Seahorse XF24 Analyzer. Maximum respiration was determined by sequential addition of oligomycin, FCCP, Rot/AA, respectively. b Changes in mitochondrial complex I activity in MAZ overexpression knockdown melanoma cell lines detected by UV spectrophotometry. c Microplate reader detects changes in ATP content in MAZ overexpression or knockdown melanoma cell lines. d Mechanistic modeling MAZ-targeted regulation of NDUFS3 in melanoma. The experimental data were normalized. Student’s t-test was used to analyse the experimental results of the MAZ-OE and MAZ-KD groups with their control groups. (mean ± SEM, n = 3). (****p < 1e-4, ***p < 2e-4, **p < 2e-3, *p < 0.05).

Back to article page