Fig. 4: Enhancement of polycomb repressive complex 2-mediated H3K27me3 in the ovaries of infertile Abcg5/g8 knockout mice.
From: Dietary phytosterols induce infertility in female mice via epigenomic modulations
a The top 10 upstream binding factors enriched in downregulated transcripts in ovaries from infertile Abcg5/g8 knockout mice are listed. ChIP-Atlas was used for enrichment analysis. The bar charts indicate the p value for enrichment, with the colors indicating polycomb repressive components (red: PRC2 component; blue: PRC1 component) or not (white). b Protein-protein interaction networks based on the STRING database (https://string-db.org) for the top 10 upstream binding factors listed in (a). c H3K27me3 levels standardized to the total histone H3 levels in the ovaries of each mouse group [CD-fed WT (n = 4); CD-fed KO (n = 5); VOFD-fed KO (n = 7)]. Bar graphs represent the mean ± S.D. The dots on the bar graphs represent each individual data point. *p < 0.05, significantly different using Sidak’s multiple comparisons test compared with CD-fed WT mice. N.S. not significantly different. d Schematic illustration of the putative mechanisms of phytosterol (PS)-mediated follicle growth suppression and egg quality reduction.
