Fig. 3: Quantitative analysis of organoid growth and viability. | Communications Biology

Fig. 3: Quantitative analysis of organoid growth and viability.

From: Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays

Fig. 3

Image-based quantification of organoid growth rate based on the Viability (V) metric, normalized to timepoint 0 (mean ± SD, n = 3) for A vehicle control and (B) positive control staurosporine. C Representative images of dying organoids. The upper panel displays the overlap of the raw brightfield and green fluorescence images that mark dead cells using cytotox green. The lower panel displays the overlay with the following segmentation and masking: Magenta = label-free organoid segmentation; Green = green fluorescence; Yellow = label-free death organoid segmentation. Scale bar: 75 μm.

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