Fig. 4: The loss of CTNNA3 leads to apoptosis.
siRNAcontrol non-targeting pool of small interfering RNAs, siRNACTNNA3 siRNA against CTNNA3, siRNAfortilin siRNA against fortilin, IB immunoblot, α-CTNNA3 anti-CTNNA3 antibody (Ab), α-fortilin anti-fortilin Ab, NA no knockdown target, A.U. arbitrary unit. a–c JESS ™ Western blot analysis of CTNNA3 and fortilin protein levels following siRNA treatments. a The total cell lysates from 293T cells transfected by siRNAcontrol, siRNACTNNA3, siRNACTNNA3/fortilin, or siRNAfortilin were subjected to JESS™ western blot analysis using α-CTNNA3 (upper panel) and α-fortilin (middle panel) Abs while total proteins were visualized and used as the loading control. b The fortilin expression level (in A.U.) was calculated by dividing the area under the curve (AUC) of the fortilin signals by that of corresponding total protein signals. c The CTNNA3 expression level (in A.U.) was calculated by dividing the AUC of the CTNNA3 signals by that of corresponding total protein signals. d Trypan blue positivity (in A.U.) of 293T cells transfected by siRNAcontrol, siRNACTNNA3, siRNACTNNA3/fortilin, or siRNAfortilin. e Activated caspase 3 levels (in A.U.) of 293T cells transfected by siRNAcontrol, siRNACTNNA3, siRNACTNNA3/fortilin, or siRNAfortilin. f DNA fragmentation levels (in A.U.) of 293T cells transfected by siRNAcontrol, siRNACTNNA3, siRNACTNNA3/fortilin, or siRNAfortilin. d–f The values indicate fold-change of the respective levels of the siRNACTNNA3, siRNACTNNA3/fortilin, and siRNAfortilin-treated cells relative to that of siRNAcontrol-treated cells. Data are expressed as means ± s.d. (n = 3 biological replicates), and they were analyzed by one-way ANOVA and Tukey–Kramer tests.
