Fig. 6: Ebony affects normal development in the Queensland fruit fly.
From: Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies

We performed genetic crossings to evaluate whether ebony-null alleles would impose fitness costs over the development of B. tryoni expressed in terms of (a) relative fecundity (no. of eggs / no. of eggs in control experiments), (b) hatchability (no. of larvae / no. of eggs), (c) pupation (no. of pupae / no. of larvae), (d) adult eclosion (no. of fully emerged adults / no. of pupae), (e) partial adult eclosion (no. of partially emerged adults / no. of pupae), and (f) adult deformity (no. of deformed adults / no. pupae). Dots represent data collected from independent replicates (n = 3), colors represent the pupae phenotype of their F1 offspring, and letters indicate significant differences (one-way ANOVA followed by Tukey’s HSD). The mean ± SD are shown in a gray dot and error bars, respectively. ctr = control crossings between wt flies, test = test crossings between Bt-ebony males and wt females, rec = reciprocal crossings between wt males and Bt-ebony females, and e = crossings between Bt-ebony flies.