Fig. 1: Detection of migrasomes in glioblastoma (GBM).
From: Glioblastoma-derived migrasomes promote migration and invasion by releasing PAK4 and LAMA4
a Confocal images of LN229 cells transfected with TSPAN4-GFP. Scale bar, 10 μm. b Ten cells were randomly selected from each of the three independent dishes, and the number of migrasomes and the length of retractile fibers formed per cell were quantified. c Transmission electron microscopy (TEM) image of LN229 cells. Scale bar, 2 μm. Boxed regions are enlarged in the small panels. Scale bar, 200 nm. d LN229 cells were grown on cover slips and observed by field emission scanning electron microscope (SEM). Scale bar, 5 μm. Boxed regions are enlarged in the small panels. Scale bar, 1 μm. e Migrasome Formation. Time-lapse images were acquired with a confocal microscope after LN229 cells transfected with TSPAN4-GFP were cultured for 6 h. Scale bar, 10 μm. f The U87, T98, U251 and U373 cells were stained with wheat germ agglutinin (WGA) and subsequently visualized. Scale bar, 20 μm. g The number of migrasomes and the length of retractile fibers in each field of view in 1 f were quantified.
