Fig. 3: TSPAN4 regulates the migration and invasion of GBM cells.
From: Glioblastoma-derived migrasomes promote migration and invasion by releasing PAK4 and LAMA4
a LN229 cells were transfected with lentivirus, and the abundance of TSPAN4 was analyzed using a Western blot. GAPDH was used as a loading control. ***P < 0.001. b WGA was used to label migrasomes in NC and Kd-TSPAN4 cells, which were then imaged by confocal microscopy. Scale bar, 50 µm. Boxed regions are enlarged in the small panels. Scale bar, 20 µm. c The number of migrasomes from 3b was quantified. ****P < 0.0001. d Representative images of the wound-healing assay using NC and Kd-TSPAN4 cells are presented. The quantified scratch coverage was analyzed. Data are presented as the mean percentage of control (0 h). **P < 0.01, ****P < 0.0001. e Cells that adhered to the underside of the Transwell membrane were stained with crystal violet. Scale bar, 50 μm. The number of migrating and invading cells were quantified. ****P < 0.0001. f Representative hematoxylin and eosin-stained sections of mouse brain after inoculation with NC or Kd-TSPAN4 cells, scale bar, 1 mm; The junction between normal and tumor tissue is enlarged in the small panels, scale bar, 200 μm. The tumor area in the mouse brain was quantified using ImageJ 1.53t software, **P < 0.01.
