Fig. 5: HE4-ANXA2 binding enhances the profibrotic phenotype in epithelial cells and promotes lung fibroblast activation. | Communications Biology

Fig. 5: HE4-ANXA2 binding enhances the profibrotic phenotype in epithelial cells and promotes lung fibroblast activation.

From: Human epididymis protein 4-annexin II binding promotes aberrant epithelial-fibroblast crosstalk in pulmonary fibrosis

Fig. 5

A Western blot analysis of fibronectin, collagen I and α-SMA expression in Beas-2B cells transfected with NC siRNA or ANXA2 siRNA followed by PBS or 50 ng/ml rhHE4 stimulation. n = 3 biologically independent experiments. B Western blot analysis of fibronectin, collagen I, and α-SMA expression in MRC-5 cells transfected with NC siRNA or ANXA2 siRNA followed by PBS or 20 ng/ml rhHE4 stimulation. n = 3 biologically independent experiments. C qRT-PCR and western blot analysis of ANXA2 expression in Beas-2B or MRC-5 cells with different concentration of rhHE4. n = 3 biologically independent experiments. D Western blot analysis of ANXA2 expression in lung homogenates of mice. n = 6 biologically independent animals. ns, not significant. *p < 0.05, **p < 0.01. Data are represented as means ± SEM.

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