Fig. 4: Genome-wide characterization of PSSP.

A Number of PSSP identified in M. incognita and the proportion of PSSP verified by proteomics. B Distribution of exon number in genes encoding PSSP. C Length distribution of PSSP in M. incognita. D–F Detection of transcripts for PSSP (Mi_25001) using in situ hybridization (ISH) in M. incognita adult females. D No ISH signal observed in the sense probe. Bar = 100 µm. E, F) ISH signal detected in the esophageal gland (G) of M. incognita J3/J4 stage using digoxingenin-labeled antisense probe. Bar = 100 µm.