Fig. 4: Production of autoantibodies against antigens of spermatocytes/spermatids and penetration of IgG into seminiferous tubules in Cldn11−/− mice. | Communications Biology

Fig. 4: Production of autoantibodies against antigens of spermatocytes/spermatids and penetration of IgG into seminiferous tubules in Cldn11−/− mice.

From: Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factor

Fig. 4

a Immunohistochemistry of wild-type testis sections using serum from a Cldn11+/− mouse as a primary antibody. Stages of spermatogenesis are shown by Roman numerals. b Immunohistochemistry of wild-type testis sections using serum from a Cldn11−/− mouse (Cldn11−/−_1) and anti-SCP3 antibody (b1), or serum from a Cldn11−/− mouse (Cldn11−/−_2) and PNA lectin (b2). Stages of spermatogenesis are shown by Roman numerals. c Western blotting of wild-type testis lysates with serum from a Cldn11+/− mouse and sera from Cldn11−/− mice used in (b). A black arrowhead and white arrowheads indicate testicular antigens detected by sera in (b1) and (b2), respectively. *IgG heavy chains. **IgG light chains. d Immunohistochemistry of frozen testis sections from Cldn11+/− mice using anti-mouse IgG and anti-CLDN11 antibodies. e Immunohistochemistry of frozen testis sections from Cldn11+/− and Cldn11−/− mice using anti-mouse IgG antibody. f Tracer assay. Dextran (150 kDa) conjugated to a fluorescent dye was injected into the interstitium of testes from Cldn11+/− and Cldn11−/− mice. Scale bars: 50 μm (a, b, e, f) and 10 μm (d).

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