Fig. 5: Extended field of view (200 µm × 200 µm × 3.2 mm) volumetric imaging (after motion correction and surface flattening) from the dorsal forearm of a 29-year-old male volunteer with the excitation wavelength of 880 nm. | Communications Biology

Fig. 5: Extended field of view (200 µm × 200 µm × 3.2 mm) volumetric imaging (after motion correction and surface flattening) from the dorsal forearm of a 29-year-old male volunteer with the excitation wavelength of 880 nm.

From: Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view

Fig. 5

a 3D image of the motion-corrected volume. b Orthogonal view of the 3D image in the yz and xy plane. The dashed yellow lines indicate the sectioning position. The three dashed rectangular boxes from left to right correspond to the position of (c–e). c The zoomed-in orthogonal view of a sweat gland with only the TPF channel (left rectangular box in b). The arrows point to a spinal sweat duct. d The zoomed-in orthogonal view of a hair follicle (central rectangular box in b). e The zoomed-in orthogonal view of a subvolume (right rectangular box in b). f Vertical plane image of the volume with only TPF and SHG channels. g 3D image of the surface flattened volume. h Vertical plane image of the surface flattened volume. i–k Horizontal plane image of the surface flattened volume at different depths. Color is coded in green for RCM, red for TPF, and magenta for SHG. Color is coded in green for RCM, red for TPF, and magenta for SHG. Scale bar 100 µm.

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