Fig. 7: In vivo volumetric multimodality microscopy imaging of a vitiligo lesion under 880 nm fs laser excitation (contrast-enhanced).

a An image of the 3D volumetric data cube of a dimension of 200 µm × 200 µm × 8 mm encompassing the transition zone from the pigmented area (right side) to the non-pigmented area (left side). b An xy plane image sectioned at the depth of 28 µm. c A yz plane image that is sectioned in the middle of the volume. d and (e) are zoomed views of a rectangular region selected (orange rectangle) in the non-pigmented area in (b) and (c). The yellow dashed line in (d) and (e) shows the corresponding section plane position of (e) and (d). f and (g) are zoomed views of a rectangular region selected (green rectangle) in the pigmented area in (b) and (c). The yellow dashed line in (f) and (g) shows the corresponding section plane position of (g) and (f). The red arrows in f point to two example pigmented cells. The black arrow in (e) points to the undulation traces of motion. Color is coded in green for RCM, red for TPF, and magenta for SHG. Scale bar: 100 µm.