Fig. 3: Example of extracellular recording from the connective containing AN1 and AN2 axons (top trace) in response to a 6 kHz sound pulse pf 20 ms (lower trace).

AN2 action potentials (marked with stars) were large enough to be identified individually. AN1 action potentials were often obscured by other neural activity. Minimum AN1 latency was assumed to be 10 ms, and AN1 activity was measured by comparing the root mean square (RMS) amplitude from for 50 ms post-latency with the RMS amplitude for 50 ms pre-latency (data available at Dryad Deposition).