Fig. 3: Induction of TNF-α by macrophages upon exposure to diverse DNA.
From: Staphylococcus aureus thermonuclease NucA is a key virulence factor in septic arthritis
a Stimulation of mouse macrophage RAW 264.7 cells with JE2Δlgt gDNA, mammalian gDNA, ODN2006 (CpG oligonucleotide), and ODN2137 (GpC dinucleotides) at varying concentrations for 18 h, followed by TNF-α measurements. b Agarose gel analysis showed the degradation of JE2Δlgt gDNA by gradually decreasing NucA concentration. c Undigested and digested JE2Δlgt gDNA were incubated with RAW 264.7 cells for 18 h. Released TNF-α in cellular supernatants was quantified by ELISA. Data represent the mean ± SEM from three independent experiments; ns(not significant), p > 0.05; *p < 0.05; ****p < 0.0001, one-way ANOVA with Dunnett’s posttest.
