Fig. 4: Induction of cytokines by various host cells upon exposure to live JE2, JE2Δnuc1, JE2Δlgt, and JE2Δnuc1Δlgt.
From: Staphylococcus aureus thermonuclease NucA is a key virulence factor in septic arthritis
The PBS-washed bacteria were incubated with (a) RAW 264.7 cells at an MOI = 30, (b) SAOS-2 cells at an MOI = 3, and (c) with neutrophils at an MOI = 50. Cellular supernatants were collected after 18 h for RAW 264.7 and SAOS-2 cells, and after 5 h for neutrophils to measure various cytokines by ELISA assay. For neutrophils, the experiments were displayed from n = 10 donors. Triplet experiments were conducted; error bars indicate ± SEM; not significant, p > 0.05; *p < 0.05; **p < 0.01; and ****p < 0.0001, one-way ANOVA with Dunnett’s posttest.
