Fig. 1: The reducing of the m6A methylase METTL3 was associated with increased risk of vascular calcification. | Communications Biology

Fig. 1: The reducing of the m6A methylase METTL3 was associated with increased risk of vascular calcification.

From: METTL3 obstructs vascular smooth muscle cells osteogenic reprogramming by methylating Runx2 in chronic kidney disease

Fig. 1

A Serum RNA m6A levels in CKD patients with VC (n = 6) or without VC (n = 12). B Serum RNA m6A level in subgroups defined by calcification Agatston score (n = 6 per group). C Correlation between serum RNA m6A level and calcification Agatston score from CKD patients with coronary arteries calcification. D Relative mRNA expression of m6A enzymes in serum from patients with CKD with VC or without VC (n = 6 per group). E RT-qPCR analysis of METTL3 mRNA expression level in subgroups defined by calcification Agatston score (n = 20 for without VC; n = 20 for mild; n = 22 for severe). F Correlation matrix of METTL3 and calcification factors. G Receiver operating characteristic (ROC) curve for detection of calcification based on METTL3. H Representative von Kossa staining and immunofluorescence for METTL3 in radial arteries slides from CKD patients with VC or without VC (n = 6 per group). **p < 0.01, ***p < 0.001. Statistical significance between the groups was determined by unpaired two-tailed student’s t tests and one-way ANOVA. VC vascular calcification, NS not significant, Pi phosphate, Ca calcium, ALP alkaline phosphatase, iPTH intact parathyroid hormone, CACs coronary artery calcification score.

Back to article page