Fig. 5: Predicted hotspot residues by Discovery Studio and growth of these mutants under various concentrations of L-canavanine.

a Mutated residues in the LysE structural model. b Growth of mutants in the MA medium containing various concentrations of L-canavanine. After being cultured at 37 °C for 24 h, the growth was monitored by measuring the OD600nm values. Data are from n = 3 independent experiments, Error bars represent SEM.