Table 2 Kinetic parameters of reactions catalyzed by McyI and its mutants
From: Structural insights into the catalytic mechanism of the microcystin tailoring enzyme McyI
McyI | WT | R249M | R249A | R166M | R166A | D173A | S86A | Y26F |
|---|---|---|---|---|---|---|---|---|
KM (μM) | 55.83 ± 7.29 | ND | ND | 162.90 ± 35.66 | 286.51 ± 16.20 | ND | ND | 72.86 ± 3.57 |
Vmax (μmol/min) | 41.53 ± 1.86 | ND | ND | 34.01 ± 4.31 | 71.78 ± 3.22 | ND | ND | 52.66 ± 0.97 |
