Fig. 2: Ciliary defects promote actomyosin contraction.
From: Primary cilia prevent activation of the cGAS-STING pathway during mouse decidualization
A F-Actin immunofluorescence, quantification of fibers length and F-Actin intensity in uterine stromal cells transfected with siScr or siTMEM67 for 24 h and further induced in vitro decidualization for 48 h. Scale bars, 15 μm. B Western blot analysis and quantification of RhoA, p-MLC2, and TMEM67 after stromal cells were transfected with siScr or siTMEM67 for 24 h and further induced in vitro decidualization for 48 h. C Western blot analysis and quantification of RhoA and p-MLC2 in stromal cells under in vitro decidualization for 2 and 3 days. D F-Actin and p-MLC2 immunofluorescence in uterine stromal cells. Scale bar, 25 μm. All images were representative of three biologically independent experiments. All data were presented as means ± SD from three biologically independent experiments. The two-tailed Student’s t-test was used for comparing two groups. One-way ANOVA test was used for comparing more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
