Fig. 5: RhoA-MLC2 signaling inhibits ciliogenesis.
From: Primary cilia prevent activation of the cGAS-STING pathway during mouse decidualization
A F-Actin immunofluorescence, quantification of fibers length and F-Actin intensity after uterine stromal cells treated with C3 (0.5 μg/ml) under in vitro decidualization (E + P) for 48 h. Scale bars, 25 μm. B Arl13b and γ-tubulin immunofluorescence, the percentage of ciliated cells, and cilia length in mouse stromal cells treated with C3 (0.5 μg/ml) with or without in vitro decidualization for 48 h. Scale bars, 15 μm. Inset scale bars, 5 μm. C Western blot analysis and quantification of IFT88, Arl13b, AcTub, and p-MLC2 after stromal cells were treated with C3 (0.5 μg/ml) with or without in vitro decidualization for 48 h. D Relative mRNA abundances of Prl8a2 and Prl3c1 after stromal cells were treated with C3 (0.5 μg/ml) with or without in vitro decidualization for 48 h. E F-Actin immunofluorescence, quantification of fibers length and F-Actin intensity in mouse stromal cells treated with Y27632 (40 μM) with or without in vitro decidualization for 24 h. Scale bars, 25 μm. F Arl13b and γ-tubulin immunofluorescence, the percentage of ciliated cells, and cilia length in mouse stromal cells treated with Y27632 (40 μM) with or without in vitro decidualization for 24 h. Scale bars, 15 μm. Inset scale bars, 5 μm. G Western blot analysis and quantification of IFT88, Arl13b, AcTub, and p-MLC2 after stromal cells were treated with Y27632 (10, 20, and 40 μM) with or without in vitro decidualization for 24 h. H Relative mRNA abundances of Prl8a2 and Prl3c1 in mouse stromal cells treated with Y27632 (40 μM) with or without in vitro decidualization for 24 h. All images were representative of three biologically independent experiments. For violin plots, the solid lines indicated the median and the dotted lines indicated the first and third quartiles. Other data were presented as means ± SD from three biologically independent experiments. The two-tailed Student’s t-test was used for comparing two groups. One-way ANOVA test was used for comparing more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
