Fig. 7: RhoA-MLC2 signaling activates the cGAS-STING pathway.
From: Primary cilia prevent activation of the cGAS-STING pathway during mouse decidualization
A dsDNA immunofluorescence and the percentage of dsDNA-colocalized micronuclei after stromal cells were treated with C3 (0.5 μg/ml) under in vitro decidualization (E + P) for 48 h. Scale bars, 25 μm. Inset scale bars, 10 μm. B Picogreen immunofluorescence and the percentage of picogreen-colocalized micronuclei after stromal cells were treated with C3 (0.5 μg/ml) under in vitro decidualization for 48 h. Scale bars, 25 μm. Inset scale bars, 10 μm. C dsDNA and cGAS immunofluorescence, and the percentage of cGAS-colocalized micronuclei after stromal cells were treated with C3 (0.5 μg/ml) under in vitro decidualization for 48 h. Scale bars, 25 μm. Inset scale bars, 10 μm. D Western blot analysis and quantification of cGAS, p-Sting, p-TBK1, and p-IRF3 after stromal cells were treated with C3 (0.5 μg/ml) under in vitro decidualization for 48 h. E dsDNA immunofluorescence and the percentage of dsDNA-colocalized micronuclei after stromal cells were treated with Y27632 (40 μM) under in vitro decidualization for 24 h. Scale bars, 25 μm. Inset scale bars, 10 μm. F Picogreen immunofluorescence and the percentage of picogreen-colocalized micronuclei after stromal cells were treated with Y27632 (40 μM) under in vitro decidualization for 24 h. Scale bars, 25 μm. Inset scale bars, 10 μm. G dsDNA and cGAS immunofluorescence, and the percentage of cGAS-colocalized micronuclei after stromal cells were treated with Y27632 (40 μM) under in vitro decidualization for 24 h. Scale bars, 25 μm. Inset scale bars, 10 μm. H, I Western blot analysis and quantification of cGAS, p-Sting, p-TBK1, and p-IRF3 after stromal cells were treated with Y27632 (10, 20 and 40 μM) under in vitro decidualization for 24 h. All images were representative of three biologically independent experiments. All data were presented as means ± SD from three biologically independent experiments. The two-tailed Student’s t-test was used for comparing two groups. One-way ANOVA test was used for comparing more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
