Fig. 8: Recovery sleep reduces sleep deprivation (SD)-induced male reproductive issues. | Communications Biology

Fig. 8: Recovery sleep reduces sleep deprivation (SD)-induced male reproductive issues.

From: Sleep deprivation impacts the immunological milieu of epididymis leading to low sperm quality in rats

Fig. 8: Recovery sleep reduces sleep deprivation (SD)-induced male reproductive issues.

A Hematoxylin and eosin (H&E) staining of cauda epididymis sections from the control and recovery sleep group rats, showing no significant change in sperm density within the lumen. Scale bars: 200 µm. B, C Histograms of sperm concentration and motility in control and recovery sleep group rats. Data are presented as the mean ± standard deviation values (3 replicates per group). Statistical analysis was performed with an unpaired t-test. ns > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, not significant. D, E Principal component analysis (PCA) and Spearman’s rank correlation coefficient analysis of RNA sequencing data from the caput, corpus, and cauda epididymis of control and recovery sleep group rats (3 replicates per group).

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