Fig. 3: Identification of the interaction between IGF2BP3 and NUDT21 in OC.

A Venn diagram of NUDT21 IP-MS data with or without RNaseA, the eukaryotic RBP database (EuRBPDB) and the RBP database for tumorigenesis (RBPTD). B Co-IP assay of NUDT21 and IGF2BP3 in A2780 OVCAR3 and SKOV3 cells. C–F PAS-seq analysis of NUDT21-knockdown OVCAR3 cells; density scatter plots of long and short genes (C); and boxplot of the proximal and distal PASs uses (D). KEGG enrichment analysis of long genes (E) and short genes (F). G and H Volcano plot of IGF2BP3-binding genes identified by RIP-seq (G) and KEGG enrichment analysis of these genes (H). I and J Volcano plot of NUDT21-binding genes identified by RIP-seq (I) and KEGG enrichment analysis of these genes (J). K Venn diagram of the candidate target genes screened via meRIP-seq, NUDT21 PAS-seq, NUDT21 RIP-seq, and IGF2BP3 RIP-seq data. L Information on four candidate target genes from 56 genes related to cancer and APA. M UCSC browser showing the long and short isoforms of SPTBN1, in which the proximal polyA site (pPAS) is in the 32 (35 total) introns. N PAS-seq data for SPTBN1 genes with NUDT21 knockdown. The proximal and distal PASs were marked by colored boxes, and the locations are labeled at the bottom. O MeRIP-seq results showing enrichment of m6A sites on intron 32 of SPTBN1.