Fig. 5: Functions of SPTBN1 isoforms in OC cells in vitro and in vivo. | Communications Biology

Fig. 5: Functions of SPTBN1 isoforms in OC cells in vitro and in vivo.

From: IGF2BP3 recruits NUDT21 to regulate SPTBN1 alternative polyadenylation and drive ovarian cancer progression

Fig. 5

A Western blot analysis of total, long, and short isoform SPTBN1-knockdown cells. B–D CCK-8 (B) (n = 4), colony formation (C) (n = 3) and Transwell (D) (n = 3) assays of A2780, OVCAR3, and SKOV3 cells infected with SPTBN1 total, long, or short isoform knockdown or negative control. E Gross xenograft model anatomy and tumor volume and tumor weight after subcutaneous injection of SPTBN1 total, long, and short isoform knockdown in OVCAR3 cells into nude mice (n = 5). F Intraperitoneal tumor metastasis of SPTBN1 total, long, and short isoform-knockdown OVCAR3 cells injected into nude mice; the graphs show the number of metastatic nodules (n = 5). G Western blot analysis of NUDT21-knockdown and SPTBN1 short isoform-overexpressing cells. H–J Overexpression of the SPTBN1 short isoform reversed the growth (H) (n = 4), proliferation (I) (n = 3) and metastasis (J) (n = 3) of OC cells in which NUDT21 was downregulated. *P < 0.05, **P < 0.01, ***P < 0.001.

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