Fig. 4: Identification of chromosome rearrangements in high-yield strains using PCR. | Communications Biology

Fig. 4: Identification of chromosome rearrangements in high-yield strains using PCR.

From: Enhancing recombinant protein production through Cre-loxP mediated chromosomal rearrangement evolution in Kluyveromyces marxianus

Fig. 4

A Genomic PCR for the 13 strains. The PCR results for 16 pairs of primers targeting the 16 loxP sites are arranged in separate lanes. Seven strains (4-C13, 6-C21, 6-C3, 7-C1, 4-H19, 5-H10, and 5-H14) showed no rearrangement, two strains (5-C12 and 7-H9) exhibited solely one rearrangement site, and four strains (7-C2, 6-H13, 7-H4, 7-H5) exhibited two rearrangement sites. B PCR validation for the rearrangements. For strain 7-C2, the primer pair 1-F and 15-F successfully amplified the target band, suggesting that an inversion has occurred within Chr VIII. Strains 6-H13, 7-H4, and 7-H5 showed amplification with the primer pair 7-F and 11-R, indicating a translocation between Chr III and Chr V.

Back to article page