Fig. 7: The immunoblotting data indicates that Benzbromarone destabilizes Hsp90’s client proteins. | Communications Biology

Fig. 7: The immunoblotting data indicates that Benzbromarone destabilizes Hsp90’s client proteins.

From: Benzbromarone interferes with the interaction between Hsp90 and Aha1 by interacting with both of them

Fig. 7: The immunoblotting data indicates that Benzbromarone destabilizes Hsp90’s client proteins.

A, B Dose-dependent decreasing of the level for AR in LNCaP cells and dose-dependent decreasing of the levels for AKT, CDK2, and CDK4 in LNCaP, MNNG/HOS Cl #5, MG-63, LS174T, HCT116, and HT-29 cells were observed upon the application of increased amounts of Benzbromarone. Additionally, treatment with Benzbromarone did not significantly up-regulate the levels of Hsp70/Hsc70 and HSF1. Each experiment was repeated for three times, and the results for one of the experiments are shown here. Statistical analysis was performed using Graphpad Prism 8 software (*p < 0.05; **p < 0.01; ***p < 0.001 compared to control, t-test).

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