Fig. 4: 3D-TSA reaction with FT-GO.

a Schematic diagram of an experimental procedure for testing 3D-TSA reaction with FT-GO and POD-nAbs. 1-mm-thick brain slices are infected with AAV2/PHP.eB CAG-EGFP-WPRE or CAG-tdTomato-WPRE are subjected to 3D FT-GO reaction with POD-nAbs. b 3D FT-GO reaction in 1-mm-thick brain slices with GFP1 POD-nAb. b_{1, 2} Representative images of CF647 tyramide deposition with GFP1 POD-nAb (magenta, b₁) and EGFP fluorescence (green, b₂) in the cerebral cortex. b₃ A merged image of (b₁) and (b₂). c Histograms showing the percentages of FT-GO⁺ cells in EGFP⁺ cells and EGFP⁺ cells in FT-GO⁺ cells (n = 466 cells, EGFP⁺ cells; n = 507 cells, FT-GO⁺ cells from 3 animals). d 3D FT-GO reaction in 1-mm-thick brain slices with RFP6 POD-nAb. d_{1, 2} Representative images of CF647 tyramide deposition with RFP6 POD-nAb (magenta, d₁) and tdTomato fluorescence (green, d₂) in the cerebral cortex. d₃ A merged image of (d₁) and (d₂). e Histograms showing the percentages of FT-GO⁺ cells in tdTomato⁺ cells and tdTomato⁺ cells in FT-GO⁺ cells (n = 619 cells, tdTomato⁺ cells; n = 704 cells, FT-GO⁺ cells from 3 animals). Data are represented as means ± SDs. Ctx: cerebral cortex, FT: fluorochromized tyramide, NF: native fluorescence. Scale bar: 200 µm.