Fig. 8: Inhibitory pre- and postsynaptic markers are relatively normal in Calb1Cre;Dag1cΔICD cerebellar cortex. | Communications Biology

Fig. 8: Inhibitory pre- and postsynaptic markers are relatively normal in Calb1Cre;Dag1cΔICD cerebellar cortex.

From: Distinct functional domains of Dystroglycan regulate inhibitory synapse formation and maintenance in cerebellar Purkinje cells

Fig. 8

Tissue sections from cerebellar vermis of Calb1Cre;Dag1cΔICDs (B) and littermate controls (A) were immunostained for Calbindin to visualize Purkinje cells along with the presynaptic marker VGAT (magenta) and postsynaptic GABAA receptor subunit GABAAα1 (green). The first panel shows a low-magnification view of the Calbindin channel. Subsequent panels are magnified views of the yellow outlined region in the first panel. Images are maximum projections. C Quantification of VGAT (left) and GABAAα1 (right) puncta density (per 100 μm3) for dendritic (“Molecular Layer”) and somatic compartments. Error bars are presented as mean + SEM. pVGAT_ML = 0.575, pVGAT_Soma = 0.018, pGABAAα1_ML = 0.372, pGABAAα1_Soma = 0.044. D Quantification of VGAT (left) and GABAAα1 (right) puncta size (in μm3) for the dendritic compartment. pVGAT = 0.424, pGABAAα1 = 0.719. Calb1Cre;Dag1WT N = 3 animals. Calb1Cre;Dag1cKO N = 3 animals.

Back to article page